Gene description for Sqstm1
Gene name sequestosome 1
Gene symbol Sqstm1
Other names/aliases A170
OSF-6
Osi
STAP
STONE14
p62
Species Mus musculus
 Experiment description of studies that identified Sqstm1 in Neuroblastoma cells

1
Experiment ID1631
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyWestern blotting
LC-MS
EV-TRACK -

2
Experiment ID1632
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyWestern blotting
LC-MS
EV-TRACK -

3
Experiment ID1637
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyWestern blotting
LC-MS
EV-TRACK -

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